WebOct 18, 2024 · Single-Cell pH Measurement. For intracellular pH imaging experiments, 35-mm glass-bottom imaging chambers were used with solution volume of ~1ml. ... The nature of this variability in cytosolic pH between various batches of cells is not known and necessitated calibration in the same group of cells exposed to drugs. Based on our … WebTobacco pollen tubes as cellular models for ion dynamics: improved spatial and temporal resolution of extracellular flux and free cytosolic concentration of calcium and protons using pHluorin and YC3.1 CaMeleon
Cytosolic pH regulates proliferation and tumour …
WebTo enable cytosolic pH measurements, we have developed cell-permeant forms of the pHrodo Red and pHrodo Green dyes (Figure 2). The pHrodo Red AM and pHrodo … WebThis process is accompanied by deactivation of blue coumarin emission within acidic organelles and restored blue/red dual emissions within the neutral cytosolic milieu, allowing for ratiometric fluorescent imaging of entire pH i gradients subjected by micellar nanoparticles following the endocytic transport pathway. philly woodworking
Spatiotemporal Monitoring Endocytic and Cytosolic pH …
WebMay 25, 2024 · Kansas City University of Medicine and Biosciences. Jul 2015 - Aug 20242 years 2 months. Kansas City, Missouri Area. • Lead a … WebDec 21, 2024 · Control cells with a cytosolic pH of 5.5 and 7.0 were prepared by permeabilization with 10 mg/ml digitonin (Across Organics, Geel, Belgium) as in Orij et al. [ 44 ], incubated at 30 °C/30 min in an … WebOct 23, 2024 · The reported observations for chloroplasts and cytosol were confirmed and extended in this study. The emission spectrum for AT1.03 from 463 to 696 nm in 10-d-old seedlings was scanned with 458-nm excitation ( SI Appendix, Fig. S1 ). philly wonderspace